PRESS RELEASE: There are Reports about E-Cigarettes

The CoEHAR researchers replicate the most relevant studies on the topic, demonstrating lower toxicity of nicotine-releasing electronic devices. Click here for the article.

International Replica project network validated the conclusion of the most important studies on e-cigarettes (e-cigs). It also confirmed the lower toxicity of aerosol produced by electronic cigarettes in comparison to conventional ones.

Catania, 17 December 2021 – Thousands of studies with different and often inconsistent results have so far been the primary snag to evaluate the toxicity of e-cigs.

This is an issue that has impacted Tobacco Harm Reduction internationally, prompting the CoEHAR to undertake an innovative and complex assessment.

“Validating specific and high-quality-standard studies in this field is our mission,” Riccardo Polosa, CoEHAR Founder, said. “Thanks to the study, we set an additional milestone on scientific research by defining effective methodological standards for research on e-cigarettes.”

In the last two years, the CoEHAR Replica project confirmed the results obtained from major international studies. It also validated their outcomes with additional techniques under independent conditions.

The study “Electronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to a cigarette: the Replica Project,” published in a journal of the Nature group, establishes an international standard in the evaluation of the relative toxicity of human bronchial epithelial cells exposed to cigarette smoke and aerosol from e-cigs.

“Our data validate many of the international studies on e-cigarettes,” Prof. Giovanni Li Volti, CoEHAR director and project leader of the Replica project, explained. “From now on, we can provide clear and consistent data to help disseminate correct information on harm reduction. In doing so we are also capable of unlocking new paths for effective and safer cessation programs.”

The study

International researchers of the Replica project from Italy, Greece, Oman, the US, and Serbia aimed to replicate three major international studies, testing them independently.

Exposure protocols for tests comply with international standards (ISO, CORESTA and HCI), and experimental conditions used in vitro were consistent with those of the smoker.

First, the researcher evaluated diverse smoke toxicity from conventional cigarettes and the volatile component with no nicotine. Therefore, they assess that acute toxicity is mainly induced (about 80%) by the volatile components rather than nicotine. Second, researchers exposed different cell cultures to the same amount of nicotine from different products (classic cigarettes, electronic cigarettes) to demonstrate no toxicity associated with the aerosol of electronic cigarettes.

Scientists performed a further comparison between the aerosol from e-cigs and heated tobacco products showing no cytotoxic effect on bronchial cells on both products.

“This is a fundamental step for the international research on the electronic cigarettes,” Prof. Massimo Caruso, CoEHAR researcher involved in the Replica project, stressed. “In doing so, we developed shared research standards replicable in the future to avoid the proliferation of scientific studies with inaccurate and ineffective methodologies”.

Via Santa Sofia 89 – 95123 Catania Presso Torre Biologica “F. Latteri” 11 piano 0954781124 |

CoEHAR – Center of Excellence for the acceleration of HArm Reduction

CoEHAR is a University research center that includes the existing “Centro per la Prevenzione e la Cura del tabagismo (CPCT),” as well as the clinical setting of a local CRO and a selected network of cell and molecular biology as well as microbiology laboratories. Thanks to their great expertise in the field, 85 University of Catania’s academics have just constituted the Center of Excellence for the acceleration of HArm Reduction (CoEHAR).

CoEHAR’s mission is to accelerate efforts to study and reduce health impacts and deaths from smoking locally, nationally, and globally through the use of approved pharmacological approaches, as well as innovative technology.

Harm Reduction is the principal topic of CoEHAR activity. The Center promotes a multidisciplinary and revolutionary approach to identify strategies and tools to stop smoking. Internationalization is the exchange of knowledge.

Via Santa Sofia 89 – 95123 Catania Presso Torre Biologica “F. Latteri” 11 piano 0954781124 |

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